Seth MacFarlane donated $2 million to NPR, plus $500,000 more to local radio station KPCC, The Los Angeles Times confirmed.
The donation, made on Tuesday, comes days after the "Family Guy" creator slammed Fox News and its host, Tucker Carlson.
NPR spokesperson Isabel Lara said the donation would "go to supporting local news coverage, allowing NPR and member stations to continue working together to tell the story of the whole country from everywhere in the country."
Bill Davis, the president and chief executive of KPCC's parent company, Southern California Public Radio, said he was "deeply moved by Seth’s generous contribution," adding that "this local/national partnership that is the heart of our shared public service mission."
On Saturday MacFarlane criticized Carlson's media attack following the release of the Justice Department's inspector general's report on the Hillary Clinton probe.
Taking to Twitter, McFarlane said he was embarrassed to work for Fox News, which has aired "Family Guy" since it premiered in 1998.
The remarks come as debates over Trump's "zero tolerance" immigration policy heat up.
Others have now joined MacFarlane in lambasting Fox News, including "Modern Family" creators Judd Apatow and Steve Levitan, USA Today noted.
"Let me officially join @SethMacFarlane in saying I’m disgusted to work at a company that has anything whatsoever to do with @FoxNews," Levitan tweeted.
He later added that, while he had "no problem with fact-based conservatism (such as WSJ)," he had a gripe with "@FoxNew’s 23-hour-a-day support of the NRA, conspiracy theories and Trump’s lies."
Apatow meanwhile tweeted that it was "important to speak up when your boss is the propaganda arm which promotes putting children in cages and holding them hostage so Trump can build a wall."